Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves

Available synthetic antioxidants have been reported to mutagenic and toxic effects. On the other hand, natural show their superiority as they are not or less toxic. Passiflora foetida has potential an antioxidant, but investigation of antioxidant activity P. chromatography column fraction reported. This studied aims investigate chromatographic fractions leaves. An assay using DPPH FRAP methods. The extraction was carried out by graded maceration, then fractionation chromatography. test Thin Layer Chromatography analysis performed determine chromatogram pattern. EC50 method from n-hexane extract: 129.035 µg/mL, ethyl acetate 206.398 methanol 97.453 while 67.851 extract : 68.981 58.787 µg/mL. Column activity, with FMetPF6 best percent inhibition 41.85±1.96 at concentration 25 µg/mL (DPPH), 26.03±0.84 9 (FRAP). leaves great antioxidant; both its activity. compare extracts fractions. Further various compounds in LC-MS/MS will facilitate active compound's isolation.